Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency
2015年12月15日 · Results: Here, we systematically investigate the effect of these two elements on knockout efficiency and showed that modifying the sgRNA structure by extending the duplex length and mutating the fourth thymine of the continuous sequence of thymines to cytosine or guanine significantly, and sometimes dramatically, improves knockout efficiency in ...
Optimizing sgRNA structure to improve CRISPR-Cas9 knockout …
By a systematic investigation of sgRNA structure we find that extending the duplex by approximately 5 bp combined with mutating the continuous sequence of thymines at position 4 to cytosine or guanine significantly increases gene knockout efficiency in CRISPR-Cas9-based genome editing experiments.
Ying DANG | Michigan State University, MI | MSU | Department of ...
Ying DANG | Cited by 1,022 | of Michigan State University, MI (MSU) | Read 21 publications | Contact Ying DANG
A CRISPR-based screen identifies genes essential for west-nile …
Ma H, Dang Y, Wu Y, Jia G, Anaya E, Zhang J et al. A CRISPR-based screen identifies genes essential for west-nile-virus-induced cell death. Cell Reports. 2015;12(4):673-683. doi: 10.1016/j.celrep.2015.06.049
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2015年7月28日 · Ma et al. use a CRISPR-based genome-wide knockout screening method to identify seven genes linked to ER-associated degradation (ERAD) as factors involved in West Nile virus (WNV)-induced cell death.
Ying Dang's research works | Texas Tech University Health Sciences ...
Ying Dang's 9 research works with 872 citations and 5,219 reads, including: HLA-E-restricted HIV-1-specific CD8+ T cell responses in natural infection
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