Yersinia pestis Characteristics Chart Gram stain: • Plump, Gram negative rods (1.0-2µm x 0.5µm) • Variable bipolar staining seen with Giemsa or Wright stain • Single cells or pairs and short chains Colony morphology: • Pinpoint, gray-white and translucent at 24h on BAP; 1-2 mm, gray-white to slightly yellow and opaque after 48h.
Gram Staining and Morphological Analysis of Yersinia Pestis
2024年7月11日 · Explore the Gram staining process and morphological characteristics of Yersinia pestis, including detailed cellular structure and microscopic findings.
Pasteurella, Yersinia, and Francisella - Medical Microbiology
Yersinia pestis is a small, Gram-negative coccobacillus, which frequently shows strong bipolar staining. However, pleomorphic and club-shaped forms are not unusual.
Yersinia pestis: Properties, Disease, Lab Diagnosis
Gram staining reveals the presence of pus cells and Gram-negative (pink) single or short-chained pleomorphic coccobacilli with rounded ends surrounded by a capsule. Wayson or Giemsa staining demonstrates the typical bipolar or safety pin appearance.
Morphology & Culture Characteristics of Yersinia pestis
2018年5月29日 · Gram Staining Reaction – Yersinia pestis is a Gram -ve (Negative) bacterium. In the microscopic view of Giemsa or Methylene blue-stained smear, shows bipolar staining (safety pin-like appearance) with the two ends densely stained and a clear central area.
Bipolar staining (resembling closed safety pin) may be evident with Gram stain but more apparent with Giemsa or Wayson stain Perform all additional work in a certified Class II Biosafety Cabinet
trate short chains in liquid media. May exhibit bipolar, “safety-pin” appearance that is not seen on Gram stain, may be exhibited . 35 ̊C, better growth at 25-28 ̊C. Grey-white, translucent pinpoint colonies a. 24h, usually too small to be seen. On BAP: After 48h: colonies approximately 1-2 mm in diameter, gray-
Plague and Other Yersinia Infections - The Merck Manuals
2024年6月2日 · Plague is caused by the gram-negative bacterium Yersinia pestis. Symptoms are mainly severe pneumonia or large, tender lymphadenopathy with high fever, often progressing to septicemia. Diagnosis is epidemiologic and clinical, confirmed by culture and serologic testing.
Yersinia pestis ID Flowchart . Major Characteristics: Gram stain morphology: Gram-negative rods, 0.5 x 1-2 mm . Colony morphology: Slow growing, pinpoint colonies after 24h; Colonies are 1-2mm, gray=white to slightly yellow and opaque on . BAP after 48 h; non-lactose fermenter on MAC/EMB; growing both . At 25-28 . 0. C and at 35-37 . 0. C.
Yersinia pestis- characteristics, habitat and virulence factors
2020年5月17日 · Yersinia pestis is a Gram-negative, coccobacilli, about (1.5 X 0.7) mm in size, arranged singly in short chains or in small groups. When stained with Giemsa or methylene blue, it shows bipolar staining with two ends densely stained and the central area clear.