ROSA26 - Wikipedia
ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice. [1] It was first isolated in 1991 [2] by the group of Philippe Soriano in a gene-trap mutagenesis screen of embryonic stem cells (ESCs). Over 800 knock-in lines have been created based on the ROSA26 locus according to the MGI database. [3]
ROSA26: The ‘Safe Harbor’ Locus in the Mouse Genome
The ROSA26 locus is known in the scientific community by the official name: gene trap ROSA 26 [Gt(ROSA)26Sor]. ROSA26 is a non-coding gene composed of three exons on mouse chromosome-6, a region where it is easy to insert genes. There are no known functional proteins encoded by the ROSA26 gene.
Identification and targeting of the ROSA26 locus in human …
Nov 25, 2007 · Here we report the identification of the human homolog of the mouse Rosa26 locus. We demonstrate targeting of a red-fluorescent protein (tdRFP) cDNA to this locus through homologous recombination...
How To Use Rosa26 Knock-in Mice In Research - Biocytogen
The Rosa26 locus in the mouse genome allows researchers to express genes of interest. Learn why scientists use it & other key benefits to Rosa26, today.
ROSA26 - an overview | ScienceDirect Topics
The ROSA26 allele is a defined locus on mouse chromosome 6 that has been used extensively for the targeted insertion of a wide variety of transgenes (Nyabi et al., 2009) including fluorescent protein reporters under transcriptional control of ubiquitously expressed housekeeping genes.
Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 …
Jan 16, 2016 · The Rosa26 locus on chromosome 6 is frequently used for the integration of transgene constructs to achieve ubiquitous or conditional gene expression in mice. The Rosa26 transcript is spliced into three exons and ubiquitously expressed in all cell types and developmental stages, but not translated to a protein .
Gene targeting to the ROSA26 locus directed by engineered zinc …
Dec 14, 2011 · The ROSA26 ZFN pair was generated by a modular assembly approach and consists of six zinc finger domains in the left array and four zinc finger domains in the right array that target the first intron of the ROSA26 locus.
Novel ROSA26 Cre-reporter Knock-in C57BL/6N Mice Exhibiting …
We produced a novel ROSA26 knock-in Cre-reporter C57BL/6N strain exhibiting green emission before and red after Cre-mediated recombination, designated as strain R26GRR. Ubiquitous green fluorescence and no red fluorescence were observed in R26GRR mice.
Mouse models for miRNA expression: the ROSA26 locus
This chapter provides an overview of the current methodologies used to generate ROSA26 knock-in lines and describes different approaches that exploit the ROSA26 gene to control expression of transgenes, including miRNAs, in a temporal, cell-type, and stage-specific fashion.
Rosa26 -targeted sheep gene knock-in via CRISPR-Cas9 system
Apr 11, 2016 · Rosa26 gene can encode a non-essential nuclear RNA in almost all organizations, and become a hot point of exogenous gene insertion. Here, we describe efficient, precise CRISPR/Cas9-mediated...