The authors developed a portal vein cannulation procedure for serial bleeding in the mouse, using a modified catheter containing a stainless steel stylet that is implanted directly in the portal vein.

2023年3月9日 · Therefore, we have introduced an organized and streamlined method to perform ex vivo mouse liver perfusions in the context of in situ NMR experiments. We also describe a unique application and discuss common issues encountered in these studies.

2021年5月10日 · To our knowledge, we describe here a novel portal vein cannulation technique using a s.c. implanted osmotic pump to achieve continuous portal vein infusion in mice. We first administered the microbial metabolite trimethylamine (TMA) over 4 weeks, during which increased peripheral plasma levels of TMA and its host liver-derived cometabolite ...

Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of...

Portal vein infusion of AAV-FIX in mice can give weak antibody response to the viral particles, which can appear in the peripheral blood days after gene transfer. Mice typically produce immunoglobulins IgG1 or IgG2a. Specific assays have been …

2022年3月9日 · The greatest factor in the reproducibility of ex vivo hepatic perfusion is the quality of surgery. Therefore, we have introduced an organized and streamlined method to perform ex vivo mouse liver perfusions in the context of in situ NMR experiments. We also describe a unique application and discuss common issues encountered in these studies.

2019年5月1日 · Pre-hepatic (non-cirrhotic) PHT is mostly caused by portal vein thrombosis while intrahepatic (sinusoidal) PHT caused by liver cirrhosis is by far the most common cause of PHT in patients [15].

Portal blood flow in normal mice ranged between 1.6 to 2.3 ml/min. Flow in the common hepatic artery ranged from 0.10 to 0.35 ml/min. Portal vein pressure in normal animals was in the wide range from 4.4 to 11.2 cmH 2 O with a mean value of 8.09 ± 2.47 cmH 2 O (Table 1). This wide range may lead to small but not significant differences when ...

2021年6月29日 · A study in JCI Insight describes a novel portal vein cannulation technique using a s.c. implanted osmotic pump for continuous portal vein infusion in mice. The method was used to...

Portal vein delivery of gene therapy vector to a mouse. (a) Displacement of intestinal tract and pancreas to gain access to portal vein. (b) Exposure of portal vein. The area...