Principles of genetic circuit design | Nature Methods
2014年4月29日 · This Review introduces tools to build transcriptional circuits and explains how the choice of different tools can affect circuit behavior and how its operation can be affected by the cellular host.
Engineered gene circuits - Nature
2002年11月14日 · These developments have signalled the emergence of a gene circuit discipline, which provides a framework for predicting and evaluating the dynamics of cellular processes.
Engineering a synthetic gene circuit for high-performance …
2024年4月17日 · Here, we focussed on an alternative strategy using synthetic gene circuits to mitigate leakiness while maximising induced expression, without modifying neither the TF nor the promoter.
Synthetic gene circuits for the detection, elimination and ... - Nature
2018年6月11日 · This Review discusses the main diagnostic and therapeutic applications of synthetic gene circuits, and their translational prospects.
Synthetic memory circuits for stable cell reprogramming in plants
2022年7月4日 · Here we present a suite of functional recombinase-based gene circuits for use in plants. We first established a range of key gene circuit components compatible with plant cell functionality.
The art of modeling gene regulatory circuits - Nature
2024年5月29日 · Here, we present some fundamental considerations to develop and analyze a model of a gene regulatory circuit of interest, either representing a natural, synthetic, or theoretical system.
Genetic circuit engineering - Latest research and news | Nature
2024年10月9日 · Genetic circuit engineering is the synthesis of unnatural DNA segments encoding protein or RNA molecules that control each other’s levels. Genetic circuit engineering is the bottom-up, local ...
Foundations for the design and implementation of synthetic …
2012年5月18日 · This paper provides an integrated synthetic gene circuit using both synthetic and endogenous modules for a proof-of-concept of a potential gene or cell-based synthetic biomedical therapy.
Designing cell function: assembly of synthetic gene circuits
2018年6月1日 · This work reports the key principles for the design of an essentially ideal synthetic oscillator; robust, persistent and tunable gene oscillations are enabled by a positive feedback module that ...
CRISPRi-based circuits to control gene expression in plants
2024年5月20日 · Our CRISPRi circuits are orthogonal, compact, reversible, programmable and modular and provide a platform for sophisticated spatiotemporal control of gene expression in plants.