Glutaredoxin 1 controls monocyte reprogramming during nutrient …
Feb 10, 2022 · We report that in chow-fed, reproductively senescent female mice but not in age-matched male mice, deficiency in the thiol transferase glutaredoxin 1 (Grx1) promotes dysregulated macrophage...
Glutaredoxin: Discovery, redox defense and much more
Jul 1, 2021 · Grx1, via its deglutathionylase activity, regulates DJ-1 activity, avoiding its degradation which correlates with neuroprotection [162]. Indeed, Grx1 knockout mice show increased glutathionylation and loss of function of DJ-1 which, in SH-SY5Y and C. elegans causes dopaminergic neuron loss, a feature associated to PD [162].
Oxidative stress-induced FABP5 S-glutathionylation protects …
Dec 7, 2021 · Here we report that the expression of deglutathionylation enzyme Grx1 is decreased in the lungs of acute lung injury mice. The acute lung injury induced by hyperoxia or LPS is significantly...
Glutathione and Glutaredoxin—Key Players in Cellular Redox
Jun 26, 2023 · Grx1 and Grx2 have both been shown to selectivity catalyze dethiolation of S-glutathionylated substrates with GSH as co-substrate. The glutaredoxin substrate specificity was determined by a carefully designed experiment using various protein mixed-disulfide substrates with glutathione- and non-glutathione-containing thiols [ 69 ].
Protein-Thiol Oxidation and Cell Death: Regulatory Role of ...
The mechanism of Grx catalysis has been characterized most extensively for human Grx1, which has been thoroughly discussed in previous reviews, along with nuances in the catalytic properties of other forms of Grx (27, 29, 30, 68, 69), and involves the removal of GSH moieties from Cys residues of proteins. This deglutathionylation occurs through ...
Real-time imaging of the intracellular glutathione redox potential
May 11, 2008 · Here we demonstrate that the fusion of roGFP2 and human glutaredoxin-1 (Grx1) allows dynamic live imaging of intracellular glutathione redox potential, EGSH, with unprecedented sensitivity and...
张雪/柯越海课题组《Nature Communications》发文揭示蛋白质谷 …
Dec 20, 2021 · 氧化应激状态下，蛋白质上的半胱氨酸残基（-SH）和谷胱甘肽（Glutathione，GSH）可以形成混合型二硫键（Protein-SSG），发生蛋白质的谷胱甘肽化，其去谷胱甘肽化过程主要由谷氧还蛋白（Glutaredoxin-1，Grx1）催化。
Chemistry and biology of enzymes in protein glutathionylation
Aug 1, 2023 · Grx1 has two GSH binding sites, a GSH scaffold site (cyan) and a GSH activator site (including K19, pink), with catalytic C23 that forms disulfide with GSH during deglutathionylation (PDB: 1B4Q). α-chloroacetamide-containing Grx1 inhibitor, CWR-J02, was developed that covalently inhibits Grx1. 7-mer peptide Grx1 inhibitor (ETRWPNT, named 2GTP1 ...
Role of Glutaredoxin1 and Glutathione in Regulating the Activity …
In this report we describe new roles for glutathione (GSH) and glutaredoxin1 (GRX1) in Cu homeostasis through their regulation of Cu-ATPase activity. GRX1 is a thiol oxidoreductase that catalyzes the reversible reduction of GSH-mixed disulfides to their respective sulfhydryls (deglutathionylation).
The thioredoxin-1 and glutathione/glutaredoxin-1 systems ... - PubMed
The thioredoxin-1 (Trx1) and the glutathione (GSH)/glutaredoxin-1 (Grx1) systems are key players in preserving cytosolic redox balance. In fact, T lymphocytes critically rely on reducing equivalents from the Trx1 system for DNA biosynthesis during metabolic reprogramming upon activation.