The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. [1] . The technical name for this procedure is cryosection. The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome. [2]

Cryosections are rapidly and relatively easily prepared prior to fixation, and they provide a good system for visualizing fine details of the cell.

To provide the necessary answers to the questions put before us in the frozen section room, there are a number of steps that must be performed without flaw. These include gross examination and sampling of the tissue, accurate embedding of the tissue, cutting and staining high-quality slides, and finally interpreting the slide (s).

2022年7月26日 · This video describes the methods for cryosectioning fixed and frozen tissue in Optimal Cutting Temperature (OCT) using a motorized cryostat. Visit this link ...

WHY SNAP FREEZE OR FIX AND CRYOPROTECT FOR FREEZING? Slow freezing can cause distortion of tissue due to ice crystal formation that replaces the architecture with a “Swiss Cheese” effect. The object is to freeze so rapidly that water does not have time to form crystals and remains in a vitreous form that does not expand when solidified.

2008年8月1日 · Although cryosections are physically less stable than paraffin- or resin-embedded sections, they are generally superior for the preservation of antigenicity and therefore the detection of antigens by microscopy.

IHC Cryosection Protocol for Fixing and Sectioning of Frozen Tissues. This technique utilizes formaldehyde-based fixation before the tissue is frozen and sectioned using a cryostat.

2018年6月9日 · The frozen section is the rapid tissue section by cooling the tissue with the help of cryostat to provide immediate report of the tissue sample. The cryostat is the instrument to freeze the tissue and also to cut the frozen tissue for microscopic section. The rapid freezing of the tissue sample converts the water into ice.

Cryosectioning, the sectioning of frozen specimens, has been an important histological tool for more than a century and continues to be extensively utilized today. However, the ability to produce high-quality sections is often a difficult process requiring extensive patience and …

2022年1月17日 · The protocols presented here describe steps for cryosectioning tissue samples to be used in light microscopy methodologies including histochemistry, enzyme immunohistochemistry, and immunofluorescence. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Cryosectioning.