Alexa Fluor 488 dye | Thermo Fisher Scientific - US
Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation designed for use with the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range.
Spectrum [Alexa Fluor 488] - AAT Bioquest
Alexa Fluor 488 is a fluorescent compound with an excitation peak at 499 nm and an emission peak at 520 nm. Other spectra of interest include: CFSE (fluorescein, CFDA, SE) , FITC (Fluorescein-5-isothiocyanate) , and iFluor 488 .
Alexa Fluor 488 Dye Profile - FluoroFinder
Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers.
Fluorochromes have unique and characteristic spectra for absorption (excitation) and emission. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength.
The Alexa Fluor Dye Series—Note 1.1 - Thermo Fisher Scientific
Spectral properties of Molecular Probes Alexa Fluor dyes. * Approximate absorption and emission maxima, in nm, for conjugates. † Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters. ‡ Extinction coefficient of the reactive dye at emission maximum in cm-1M-1.
Alexa Fluor - Wikipedia
The excitation and emission spectra of the Alexa Fluor series cover the visible spectrum and extend into the infrared. [3] The individual members of the family are numbered according roughly to their excitation maxima in nanometers.
Alexa Fluor conjugated secondary antibodies - Abcam
2023年4月27日 · Showing as yellow in the vial, Alexa Fluor® 488 is a green dye that matches the fluorescence of Cy2 and FITC. Excitation is ideally suited to 496 nm for argon ion lasers, with maximum absorption of 495 nm and maximum emission of 519nm. The quantum yield is …
Alexa Fluor 488 dye and orange-fl uorescent Alexa Fluor 546 dye, the Alexa Fluor 532 dye and its conjugates are ideal for use with 532 nm excitation sources, including the frequency- doubled Nd:YAG laser.
Alexa Fluor 488® Secondary Antibodies - Jackson Immuno
Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488.
Fluorophore Excitation and Emission Data 526SP 526 nm. IIIIIIII Wavelength (nm) 350 400 450 500 550 600 650 700 For more information on Bio-Rad imaging products, contact your local Bio-Rad representative. In the US, ... Alexa Fluor 488 Molecular Probes General conjugated label Good 495 nm 520 nm 530DF30 530DF60
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