Isomerase - an overview | ScienceDirect Topics
Glucose isomerase catalyzes the reversible isomerization of glucose to fructose, which is much sweeter than glucose. This transformation represents the major commercial application of immobilized biocatalysts in industry. The glucose isomerase enzyme is an intramolecular oxidoreductase, which is able to interconvert aldoses and ketoses.
Isomerase - an overview | ScienceDirect Topics
1999年3月4日 · Isomerase. In subject area: Neuroscience. An isomerase is an enzyme that catalyzes the process of isomerization, which involves transforming a molecule into a different isomere. It accelerates the switching between different conformations of molecules, such as cis and trans forms, to affect protein conformation.
Isomerase - an overview | ScienceDirect Topics
Another relevant isomerase in sugar modification is sucrose isomerase (EC 5.4.99.11), used in the production of isomaltulose (β-D-fructofuranosyl-1,6-α-D-glucopyranoside), by rearranging the α-1,2 bond between glucose and fructose into an α-1,6 bond. The resulting disaccharide, commercialized under the trade name Palatinose™, is half as ...
Biochemical characterisation of triose phosphate isomerase from …
2013年11月1日 · Triose phosphate isomerase (TPI) catalyses the interconversion of dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, a reaction in the glycolytic pathway. TPI from the common liver fluke, Fasciola hepatica, has been cloned, sequenced and recombinantly expressed in Escherichia coli. The protein has a monomeric molecular mass of ...
Isomerase Reaction - an overview | ScienceDirect Topics
The monomeric 33-kDa enzyme catalyzes the isomerase reaction in the presence of a divalent metal cation such as Mg 2 + or Mn 2 +. 54, 55 Yeast IPP isomerase is completely inhibited by thiol-blocking reagents such as iodoacetamide, suggesting that a cysteine residue is involved in the catalytic mechanism. 56 Similar IPP isomerase activity in E.
Xylose Isomerase - an overview | ScienceDirect Topics
Xylose isomerase (d -xylose ketol-isomerase) catalyzes the isomerisation reaction of D-xylose into xylulose. This is initial step of xylose metabolism in microbial cell physiologies (Wovcha et al., 1983). Xylose isomerases are also referred to as glucose isomerases because of their capability to exchange d -glucose into d -fructose.
Isomerase - an overview | ScienceDirect Topics
The human genome has two active 3β-HSD/Δ5-4 isomerase genes: one encodes a protein predominantly expressed in the placenta, liver, breast, and brain (HSD3B1), the other a protein expressed in the gonads and adrenal cortex (HSD3B2). These two genes, each consisting of four exons each, lie 100 kb apart on band 1p13.1.
Isomerase - an overview | ScienceDirect Topics
The compound, 2-(dimethylamino)ethyl diphosphate (44), was a potent inhibitor of Claviceps IPP isomerase at pH 7.0, 80–82 inactivated the enzyme in a time-dependent manner, and formed a stable 1:1 complex with the enzyme (k off < 1.1×10 −7 s −1 and k D < 10 −10 m for Claviceps isomerase). 82 The dissociation constant for (44) with the ...
Glucose Isomerase - an overview | ScienceDirect Topics
Glucose 6-phosphate isomerase, derived from Thermus species, isomerized glucose 6-phosphate to fructose 6-phosphate. The substrate was available from starch by digestion with pullulanase of the same origin as the isomerase. 3156 Such a conversion of a starch hydrolyzate into fructose is possible on anion-exchange resins. 3157
Triosephosphate isomerase: the perfect enzyme, but how does it …
Triosephosphate isomerase catalyses the interconversion of dihydroxyacetone phosphate (DHAP) and d -glyceraldehyde-3-phosphate (d -GAP). Figure adapted from Alahuhta et al. (2008), with the permission of IUCr Journals. But, first a little more history. The structure of TIM (Banner et al., 1975) also became famous as its 8 alpha-helices ...