TaqMan® Gene Expression Assays are based on 5' nuclease chemistry, which uses a fluorogenic probe to enable the detection of a specific PCR product as it accumulates during PCR. Each assay contains: A pair of unlabeled primers

TaqMan probes are hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher Kary Mullis at Cetus Corporation, [ 1 ] and the technology was subsequently developed by Hoffmann-La Roche for diagnostic assays and by Applied Biosystems (now part of Thermo Fisher ...

TaqMan® reagents have been designed and validated on all currently available Applied Biosystems® real-time PCR instruments for consistently superior results. They are optimized for use with TaqMan® Assays and provide robust amplification under …

Use our new and improved online search tool to find Taqman Real-Time PCR Assays & Arrays for your gene expression, SNP genotyping, and MicroRNA analysis. Also get access to learning centers and additional product and service information for TaqMan qPCR Assays.

2024年6月13日 · TaqMan probes are hydrolysis probes used in quantitative PCR (qPCR) to increase specificity by utilizing the 5´–3´ exonuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization, resulting in a fluorescence signal that allows for precise quantification of the target DNA.

All products use TaqMan® probe-based chemistry and are designed for use on the suite of Applied Biosystems Real-Time PCR Systems— together the gold standard in quantitative gene expression offering the greatest sensitivity, specificity, reproducibility, and the broadest dynamic range. Table of Contents TaqMan® Gene Expression Assays 2

TaqMan Fast Advanced Master Mix enables real-time PCR in any gene expression experiment or quantitative analysis, including the following applications: • Pathogen detection

2024年9月21日 · Among these technological breakthroughs, TaqMan probes stand out as a pivotal tool for the precise and efficient monitoring of DNA amplification processes. These probes are integral to polymerase chain reaction (PCR) methodologies, enabling researchers to quantify nucleic acids with high specificity and sensitivity.

2017年11月8日 · Applied Biosystems developed TaqMan ® Gene Expression Assays, a genome-wide collection of quantitative, standardized assays for gene expression analysis by real-time quantitative RT-PCR. The TaqMan ® Assay utilizes the 5' nuclease activity of Taq DNA polymerase to cleave a fluorescently labeled probe (FAM TM -labeled MGB).

The Applied Biosystems TaqMan ® assay is a fluorogenic PCR-based system enabling real-time detection of PCR products, based on Roche Molecular Systems TaqMan® instrument. The assay utilises the 5’nuclease activity of Taq DNA polymerase to hydrolyse an internal fluorogenic probe for monitoring amplification of the DNA target (the Salmonella ...