Flow cytometric analysis of normal and reactive spleen
2004年4月23日 · Pan-T cell markers CD2 and CD3 were expressed on 38 and 31%, respectively, of normal spleen lymphocytes, and on 47 and 40%, respectively, of reactive spleen cells.
Multispectral Imaging of T and B Cells in Murine Spleen and Tumor
We compared different fixation protocols and Ag-retrieval techniques and validated the use of multiplex immunohistochemistry for detection of CD3 + CD4 + and CD3 + CD8 + T cell …
In situ engineering of mRNA-CAR T cells using spleen-targeted …
Specifically, the anti-CD3 antibody (aCD3) armed mRNA-LNPs (CD3-mRNA-LNPs) selectively targeted T cells, resulting in the production of functional and therapeutic levels of CAR T cells …
Spleen: Development, anatomy and reactive lymphoid proliferations
2021年3月1日 · Lymphoid distributions in the normal spleen. (A) CD20 highlights the B-cell follicles while CD138-positive plasma cells are predominantly lodged in the red pulp (B). CD3 …
Analysis of T cells in mouse lymphoid tissue and blood with flow ...
2021年3月19日 · Monitoring of T cell frequency and function in genetically modified mice or murine models of disease is therefore of high interest. Here, we provide a detailed protocol to …
Identification and isolation of splenic tissue-resident macrophage …
2018年9月25日 · Tissue-resident macrophages in the spleen, including red pulp and white pulp macrophages, marginal zone macrophages (MZMs) and marginal zone metallophilic …
Investigation of human spleen dendritic cell phenotype and …
2001年6月1日 · In this report, rare event flow cytometry and in situ immunofluorescence were used to study the surface phenotype and distribution of HLA-DR + CD3 − 14 − 16 − 19 − …
Expression of CD3 in spleen cells (A) and peripheral
Natural killer (NK) cells are CD3:TCR-, CD16+, CD56+ large granular lymphocytes capable of recognizing and eliminating a variety of virus-infected, malignant, and antibody-coated target …
The distribution of CD3⁺ lymphocytes and CD3 ... - ResearchGate
The spleen tissue sections were used in immunohistochemical staining for the expression of CD3 in the splenic lymphocytes. The CD3⁺ lymphocytes were stained as yellowish brown.
A diverse fibroblastic stromal cell landscape in the spleen directs ...
2022年1月7日 · We found analogous fibroblastic stromal cell diversity in the human spleen. In addition, we identify the transcription factor SpiB as a critical regulator required to support …