SLGT1 (SLC5A1) was the first cloned mammalian secondary active transporter. The first isoform was cloned from rabbit intestine in a novel way: by using functional expression of mRNA in Xenopus laevis oocytes as the screening technique. This method is now commonly called 'expression cloning' Hediger et al (1987).

2024年2月22日 · Through biochemistry experiments, we demonstrated that the spliced form of XBP1 (XBP1s) acts as a transcription factor for SLC5A1 by directly binding to its promoter region.

Expression of SLC5A1 (D22S675, NAGT, SGLT1) in hippocampus tissue. Antibody staining with HPA051805, HPA055106 and CAB015467 in immunohistochemistry.

2024年2月22日 · Through biochemistry experiments, we demonstrated that the spliced form of XBP1 (XBP1s) acts as a transcription factor for SLC5A1 by directly binding to its promoter region. Targeting this ER stress → SLC5A1 axis by either the ER stress inhibitor Rapamycin or the SGLT1 inhibitor Sotagliflozin was effective in attenuating the ER stress ...

In the present work, we confirmed that the levels of SLC5A1 mRNA and SGLT-1 protein levels were upregulated in the CFBE-dF cells compared with those in the cells of wild-type CFTR genotype (CFBE-WT) by RT-qPCR, Western blot and immunofluorescence staining (Figure 1A to …

Here, we describe an optimized methodology, based on combined use of DiI and immunofluorescence, for rapid and sensitive characterization of the structure and composition of spine synapses in ...

2022年9月16日 · To investigate their subcellular distribution in neurons, we optimized protocols for immunofluorescence staining of intracellular or plasma membrane PIPs with commercially available antibodies. Here, we describe the preparation and transfection of primary mouse hippocampal neurons in dissociated culture, followed by immunofluorescence staining ...

2019年10月16日 · Immunofluorescence analysis confirmed that SGLT1 and SGLT2 proteins remained colocalized with glucagon in islets from both obese individuals and those with type 2 diabetes (125853). Mapping Hediger et al. (1988) used a cDNA clone to probe human DNA and to map the human SLC5A1 gene.

2024年12月25日 · SLC5A1 (Solute Carrier Family 5 Member 1) is a Protein Coding gene. Diseases associated with SLC5A1 include Glucose/Galactose Malabsorption and Fructose Intolerance, Hereditary . Among its related pathways are Digestion and absorption and Disorders of transmembrane transporters .

2022年11月24日 · Here, we explore the role of glucose (SLC5A1) and inositol transporters (SLC5A3) in GBM cell migration. (2) Methods: Using immunofluorescence microscopy, we visualized the subcellular localization of SLC5A1 and SLC5A3 in …