Short hairpin RNA - Wikipedia
A short hairpin RNA or small hairpin RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi).
Short Hairpin RNA - an overview | ScienceDirect Topics
shRNAs are fabricated by RNA with hair-pin shaped entities for mRNA silencing. Their expression within the target cells is accomplished by transfection via plasmid DNA or viral vectors, like plasmids, lentivirus, retrovirus, adenovirus and adeno-associated virus.
Small Hairpin RNA - an overview | ScienceDirect Topics
Short hairpin RNA or small hairpin RNA (shRNA) is an artificial RNA molecule with a hairpin turn having a high affinity toward its target. It is processed by the RNA silencing machinery. The construct for shRNA expression is generally made under …
Expressing short hairpin RNAs in vivo | Nature Methods
2006年8月23日 · Promoter-based expression of short hairpin RNAs (shRNAs) may in principle provide stable silencing of genes in any tissue. As for all approaches that require transgene expression, safe...
Short Hairpin RNA-Mediated Gene Silencing | SpringerLink
2012年1月1日 · Since the first application of RNA interference (RNAi) in mammalian cells, the expression of short hairpin RNAs (shRNAs) for targeted gene silencing has become a benchmark technology. Using plasmid and viral vectoring systems, the transcription of …
Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of …
2010年1月1日 · Shortly after the cellular mechanism of RNA interference (RNAi) was first described, scientists began using this powerful technique to study gene function. This included designing better methods for the successful delivery of small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) into mammalian cells.
Short hairpin RNA (shRNA): design, delivery, and assessment of …
2010年1月1日 · Shortly after the cellular mechanism of RNA interference (RNAi) was first described, scientists began using this powerful technique to study gene function. This included designing better methods for the successful delivery of small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) into mammalian cells.
Second-generation shRNA libraries covering the mouse and human ... - Nature
2005年10月2日 · We generated large-scale-arrayed, sequence-verified libraries comprising more than 140,000 second-generation short hairpin RNA expression plasmids, covering a substantial fraction of all...
Cloning of short hairpin RNAs for gene knockdown in …
We have developed a system to drive expression of shRNAs by placing them under the control of the human RNA polymerase III U6 small nuclear RNA (snRNA) promoter, which normally controls...
25: RNA interference - Biology LibreTexts
2024年8月22日 · Method. siRNAs (small interfering RNAs) are short, ~19-21 basepair pieces of double-stranded RNA. They are processed by endogenous machinery in many cells, resulting in a temporary decreased expression of the target gene, usually on the scale of a few hours to a few days. siRNAs are transfected into cells, and once they degrade, expression of ...
- 某些结果已被删除