CRISPR genome editors are already being deployed in medicine and agriculture, and this Review highlights key examples, including a CRISPR-based therapy treating sickle cell disease, a more nutritious CRISPR-edited tomato, and a high-yield, disease-resistant CRISPR-edited wheat, to illustrate CRISPR’s current and potential future impacts in ...

2025年1月31日 · CRISPR, which originally evolved in bacteria as part of their immune system, is akin to a set of molecular scissors that cleaves DNA at precise locations, allowing it to excise a problem portion of a gene with help from a strand of RNA that guides it into location.

2014年11月28日 · We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism, which has set the stage for remarkable developments using this technology to modify, regulate, or mark genomic loci in a wide variety of cells and organisms from all ...

2012年6月28日 · They first showed that CRISPR—which stands for clustered regularly interspaced short palindromic repeats—could edit DNA in an in vitro system in a paper published in the 28 June 2012 issue of Science.

CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) screens are powerful tools to test the gain and loss of gene function, but their use has largely been limited to immortalized cell lines. Schmidt et al. report an optimized method that allowed them to perform genome-wide CRISPRa and CRISPRi screens on primary human T cells. This ...

2024年10月24日 · CRISPR-enabled rapid and systematic accounting of selective genetic vulnerabilities in cancerous cells (see the figure)—both in the form of cancer-type dependencies and synthetic lethal interactions—has galvanized drug developers to match new genetic observations with therapeutic agents that generate similarly selective response profiles in ...

2013年1月3日 · Bacterial and archaeal clustered regularly interspaced short palindromic repeats (CRISPR) systems rely on CRISPR RNAs (crRNAs) in complex with CRISPR-associated (Cas) proteins to direct degradation of complementary sequences present within invading viral and plasmid DNA (1–3).

This enables the model to predict how small DNA changes affect an organism’s fitness, generate realistic genome-length sequences, and design new biological systems, including laboratory validation of synthetic CRISPR systems and IS200/IS605 transposons.

2018年8月31日 · Over the past 6 years, however, transformative discoveries have shaped the CRISPR (clustered regularly interspaced short palindromic repeats) Cas (CRISPR-associated) toolbox for genetic manipulation on the basis of simpler RNA-guided DNA recognition.

The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome editing, coupled with advances in computing and imaging capabilities, has initiated a new era in which genetic diseases and individual disease susceptibilities are both predictable and actionable.