Rare cells in water samples include various pathogenic bacteria and those infected by viruses. Since preparation techniques for rare cell isolation depend on the source from where the cell samples are obtained, this article mainly focuses on isolation of rare cells from biological samples such as blood and other bodily fluids.

Having adjusted a relative high cell spike in the preceding experiments for reasons of cell loss identification and qualitatively analysis by flow cytometry, spiking quantities that simulate true rare cell frequencies may give a closer estimate on the possible LOD of the isolation/detection assay.

Various cellular biomarkers, including physical properties, dielectric properties, as well as immunoaffinities, have been explored for isolating rare cells. In this Focus article, we discuss the design considerations of representative microfluidic devices for rare cell isolation and analysis.

Abstract. Rare cell populations provide a patient-centric tool to monitor disease treatment, response, and resistance. However, understanding rare cells is a complex problem, which requires cell isolation/purification and downstream molecular interrogation – processes challenged by non-target populations, which vary patient-to-patient and change with disease.

Rare cell isolation requires special protocols that are gentle and accurate to retain a sufficient amount of healthy target cells. Learn more here.

2025年1月8日 · Programmable Enrichment via RNA FlowFISH by sequencing (PERFF-seq) isolates rare cells based on RNA marker transcripts for single-cell RNA sequencing profiling of complex tissues, with ...

2025年2月7日 · To profile rare cells, these isolation methods have typically been followed by microarray or bulk RNA sequencing 4,5, but this approach masks heterogeneity within the sorted cells.Modern advances ...

Some important applications include the detection and isolation of rare fetal cells from maternal blood for prenatal diagnosis (Cupp el af., 1984), detection of rare metastatic breast (Leslie ef a f.. 1990) or neuroblastoma (Frantz c( af., 1988) cells in bone marrow, monitoring of minimal residual disease (Ryan et al., 1984), detection of stem c...

1995年4月1日 · The analysis and isolation of previously inaccessible rare cells, such as peripheral hematopoietic stem cells, fetal cells in maternal blood, residual tumor cells or antigen-specific lymphocytes, has now become feasible through the development of new methods.

2 天之前 · The application of single-cell technologies has facilitated the prediction of cellular trajectories, the identification of rare cell populations, a more profound comprehension of malignant cell ...