Northern blotting allows one to observe a particular gene's expression pattern between tissues, organs, developmental stages, environmental stress levels, pathogen infection, and over the course of treatment.

3 天之前 · Northern blot is a laboratory analysis method used to study RNA. Specifically, purified RNA fragments from a biological sample (such as blood or tissue) are separated by using an electric current to move them through a sieve-like gel or matrix, which allows smaller fragments to move faster than larger fragments.

2021年6月4日 · Northern blot is a technique based on the principle of blotting for the analysis of specific RNA in a complex mixture. The technique is a modified version of the Southern Blotting, which was discovered for the analysis of DNA sequences.

Since northern blot uses size-dependent separation, this technique can not only determine the abundance but also the sizes of transcript of interest. It can be a very effective way to detect transcript variants of genes.

The Northern blotting procedure is straightforward and provides opportunities to evaluate progress at various points (e.g., integrity of the RNA sample and how efficiently it has transferred to the membrane).

2024年8月8日 · Northern blotting is a laboratory technique used to detect and analyze specific RNA sequences within a complex mixture, providing information about RNA length and sequence variations.

2023年4月11日 · The technique that is used in molecular biology research to study gene expression by detection of RNA or isolated mRNA in a sample is called northern blotting (RNA blotting). It is a classical method for analysis of the size and steady state level of a specific RNA in a complex sample.

2024年11月23日 · The Northern blot is a technique used in molecular biology research to study gene expression in a sample, through detection of RNA (or isolated messenger RNA ). With Northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression levels during differentiation, morphogenesis ...

Northern blotting can be used to analyze a sample of RNA from a particular tissue or cell type in order to measure the RNA expression of particular genes. This method was named for its...

In short, Northern blotting is the process of measuring or quantifying RNA, especially mRNA. The need to measure the RNA emerged to: Identify the types of protein secreted by specific tissues. And also determine what the regulating factors of the gene expression are.