The mouse model of liver ischemia and reperfusion injury has proven to be valuable for our understanding of the role that reactive oxygen and nitrogen metabolites play in postischemic tissue injury. This methods paper provides a detailed protocol for inducing partial liver ischemia followed by reper …

The mouse model of liver ischemia and reperfusion injury has proven to be valuable for our understanding of the role that reactive oxygen and nitrogen metabolites play in postischemic tissue injury. This methods paper provides a detailed protocol for inducing partial liver ischemia followed by reperfusion.

The different surgical options necessary for treating complications in the liver, ranging from liver failure to intrahepatic lesions, require a period of ischemia. Ischemia–reperfusion injury (IRI) involves two interacting phases: local ischemia injury and inflammation-mediating reperfusion injury [ 1 ].

Aging of the liver is associated with mitochondrial dysfunction and decreased intrahepatic energy content, resulting in poorer tolerance against ischemic injury. Improving intrahepatic ATP levels in old livers by glucose injection protects the old liver against ischemic injury and restores the protective effects of ischemic preconditioning.

Communications Biology - Liver zonation across a murine ischemia/reperfusion injury model is characterised and the protective effect of celastrol against I/R injury by activating HIF1α...