In situ hybridization - Wikipedia
In situ hybridization is used to reveal the location of specific nucleic acid sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation, and function of genes.
In Situ Hybridization (ISH) - National Center for Biotechnology Information
2017年11月9日 · In Situ Hybridization (ISH) is a technique that allows for precise localization of a specific segment of nucleic acid within a histologic section. The underlying basis of ISH is that nucleic acids, if preserved adequately within a histologic specimen, can be detected through the application of a complementary strand of nucleic acid to which a ...
In Situ Hybridization - National Human Genome Research Institute
4 天之前 · In situ hybridization is a laboratory technique used to localize a sequence of DNA or RNA in a biological sample. In this technique, a biological sample consisting of tissue sections, cells or chromosomes from an individual is affixed to a glass slide and then exposed to a “probe”—a small piece of single-stranded DNA tagged with a ...
In situ hybridization: Introduction to techniques, applications …
2019年9月1日 · In situ hybridization (ISH) has become a common laboratory technique used for the analysis of gene expression and for the localization of specific DNA and RNA molecules in cells. Many different methods of performing ISH have been described.
9.5: In Situ Hybridization - Biology LibreTexts
2023年7月13日 · In situ hybridization is used to reveal the location of specific nucleic acid sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation, and function of genes.
In Situ Hybridization (ISH) - SpringerLink
2022年11月25日 · In situ hybridization is the method to visualize specific DNA or RNA sequences in tissue sections, cell or chromosome preparations, and whole intact organisms. It combines molecular biological techniques with histological and cytological analysis.
Fluorescence in situ hybridization | Nature Methods
This protocol describes fluorescence in situ hybridization (FISH) of biotin- or digoxigenin-labeled probes to denatured metaphase chromosomes and interphase nuclei.
15: In situ hybridization - Biology LibreTexts
2024年8月22日 · In situ hybridization shows where in a tissue sample an RNA of a specific sequence is found or where in a set of chromosomes a DNA of a specific sequence is found.
Fluorescence In Situ Hybridization (FISH) - Nature
Today, most in situ hybridization procedures use fluorescent probes to detect DNA sequences, and the process is commonly referred to as FISH (fluorescence in situ hybridization). A...
Molecular Pathology—In Situ Hybridization - PMC - PubMed …
In situ hybridization is a method of localizing and detecting specific mRNA sequences in preserved tissue sections or cell preparations by hybridizing the complementary strand of a nucleotide probe to the sequence of interest.