2019年9月16日 · 目的： 探索肝纤维化的病理过程，明确活化的肝星状细胞对细胞外基质（ECM）的增殖、收缩和分泌作用，同时进一步分析H2O2 对肝星状细胞（HSC）凋亡的诱导途径、凋亡情况、凋亡后 ECM 代谢的变化情况。 方法： 选取 2019 年 4 月某研究院浓度为 10% 的胎牛血清 DMEM 培养 HSC-T6 作为研究对象。 胎牛血清在传代后，换无血清培养基培养。...

2022年2月15日 · In the present study, we initially induced autophagy in two immortalized HSC cells (T6 and LX2) by hydrogen peroxide (H 2 O 2) to mimic the chronic liver injuries resulting from the ROS stimuli. Next, we treated them with IL-10, autophagy-related proteins, autophagosome vesicles, and autophagic flux to evaluate the regulatory effect of IL-10 on ...

2023年2月16日 · Hypoxia and hydrogen peroxide (H2O2) accumulation form the profibrogenic liver environment, which involves fibrogenesis and chronic stimulation of hepatic stellate cells (HSCs).

2024年7月10日 · Results: In H2O2-treated HUVECs, HSC-MVs increased cell viability, reduced cytotoxicity and apoptosis, improved oxidative stress, migration, and angiogenesis, and upregulated protein expression of PI3K, AKT, MEK1/2, ERK1/2, VEGF, eNOS, and CXCR4. Conversely, TGF-β1HSC-MVs exhibited opposite effects.

Results showed that (1) HSC-MVs derived from LX-2 and HST-T6 were positive to CD90 and annexin V surface markers; (2) HSC-MVs dose-dependently improved the viability of hepatocytes in both injury models; (3) HSC-MVs dose-dependently inhibited the APAP/H 2 O 2 induced hepatocytes apoptosis and activated caspase-3 expression and leakage of LDH, AL...

2020年7月20日 · Cellular metabolism in hematopoietic stem cells (HSCs) is an area of intense research interest, but the metabolic requirements of HSCs and their adaptations to their niches during development have remained largely unaddressed. Distinctive from other tissue stem cells, HSCs transition through multiple hematopoietic sites during development.

More than 95% of H 2 O 2 -treated HepG2 cells expressed the cell cycle inhibitor, p21; >90% expressed senescence-associated heterochromatic foci, HMGI-C, confirming the induction of cell ...

超氧阴离子诱导的hsc凋亡取决于jnk激活和谷胱甘肽状态。结论活性氧（ros）在体外抑制hsc增殖并促进hsc细胞死亡。不同的ros诱导不同的细胞死亡模式。超氧阴离子诱导的hsc凋亡取决于jnk激活和谷胱甘肽状态。结论活性氧（ros）在体外抑制hsc增殖并促进hsc细胞死亡。

In vitro experiments included the induction of hepatic stellate cell (HSC-T6) activation by H 2 O 2. A cell injury model was established for HSC-T6, and the CCK-8 assay was used to determine the optimal YJSB concentration and treatment duration.

Results showed that (1) HSC-MVs derived from LX-2 and HST-T6 were positive to CD90 and annexin V surface markers; (2) HSC-MVs dose-dependently improved the viability of hepatocytes in both injury models; (3) HSC-MVs dose-dependently inhibited the APAP/H 2 O 2 induced hepatocytes apoptosis and activated caspase-3 expression and leakage of LDH, AL...