In the fields of histology, pathology, and cell biology, fixation is the preservation of biological tissues from decay due to autolysis or putrefaction. It terminates any ongoing biochemical reactions and may also increase the treated tissues' mechanical strength or stability.

Fixation of tissues can be achieved by chemical or physical means. Physical methods include heating, micro-waving and cryo-preservation (freeze drying). Heat fixation is rarely used on tissue specimens, its application being confined to smears of micro organisms.

2020年7月9日 · A quick guide on tissue fixation to obtain the best histology results. Main Points: • Freshly harvested tissue of interest should be immediately fixed to avoid degradation. • 10% Neutral Buffered Formalin (NBF) or 4% Paraformaldehyde solution …

Fixation is a complete and complex physiochemical process wherein cells and tissues are chemically fixed for further analyses. Fixatives are used for Solidification, Hardening, Optical differentiation, prevention of autolysis and tissue putrefaction.

Fixation is process in which cells or tissue are fixed in physical state and partly in chemical state so that they will with stand subsequent treatment with various reagents with a minimum loss, distortion or decomposition. Fixatives act by denaturing or precipitating proteins which then form a meshwork due to cross linking of proteins.

2023年5月1日 · Fixation: Fixation uses chemicals to preserve the structure of the tissue in its natural form and protects it from degradation by irreversibly cross-linking proteins. Although several specialized fixatives are available, Neutral Buffered Formalin is a …

Fixation preserves a sample of biological material (tissue or cells) as close to its natural state as possible in the process of preparing tissue for examination. To achieve this, several conditions usually must be met. First, a fixative usually acts to prevent autolysis.