DAPI - Wikipedia
When bound to double-stranded DNA, DAPI has an absorption maximum at a wavelength of 358 nm (ultraviolet) and its emission maximum is at 461 nm (blue). Therefore, for fluorescence microscopy, DAPI is excited with ultraviolet light and is detected through a blue/cyan filter.
DAPI (4',6-diamidino-2-phenylindole) - Thermo Fisher Scientific
We offer a number of nucleic acid stains—with various emission wavelengths—that can be used as alternatives to DAPI for imaging live cells. For live-cell staining, Hoechst 33342 dye is a popular cell-permeant nuclear counterstain.
DAPI Dye Profile - FluoroFinder
DAPI (4',6-diamidino-2-phenylindole (DAPI) is a blue-emitting fluorogenic DNA-binding dye that can be excited by the 355 nm blue laser and captured with a 450/50 nm bandpass filter. DAPI has an excitation peak at 350 nm and an emission peak at 465 nm.
Fluorochromes have unique and characteristic spectra for absorption (excitation) and emission. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. A tandem dye consists of a donor and acceptor fluorochrome molecule, placed in close proximity, allowing for energy transfer between the two.
DAPI and Hoechst Nucleic Acid Stains - Thermo Fisher Scientific
DAPI is a classic nuclear counterstain for immunofluorescence microscopy, as well as an important component of high-content screening methods requiring cell-based quantitation of DNA content and segmentation (em/ex 341/452 nm).
4',6-Diamidino-2-phenylindole, [DAPI]
The fluorescence emission spectrum of 4',6-Diamidino-2-phenylindole, [DAPI] dissolved in dimethylsulfoxide. The excitation wavelength was 310nm. The quantum yield of this molecule is 0.58 (Hà  à ¤rd, 1990). This spectrum was collected by in the summer of 1997 using a …
DAPI - AAT Bioquest
DAPI, or 4',6-diamidino-2-phenylindole, is a fluorescent compound with an excitation peak at 359 nm and an emission peak at 457 nm. It is well excited by the 405 nm violet laser or the 355 nm UV laser. The emission can be captured by a 450/50 nm bandpass filter or using the DAPI filter set configuration on fluorescence microscopes.
DAPI Nuclear Staining Dye | Bio-Rad
When bound to dsDNA, PureBlu DAPI Dye has a maximum excitation wavelength in the ultraviolet range (359 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2).
DAPI Protocol for Fluorescence Imaging - Thermo Fisher Scientific
A popular nuclear and chromosome counterstain, DAPI (4′,6-diamidino-2-phenylindole) emits blue fluorescence upon binding to AT regions of DNA. This protocol can be used for: Nucleic acid (nuclear) staining in fluorescence microscopy
a longer-wavelength fluorescence emission maximum than the DAPI/dsDNA complex (~500 nm versus ~460 nm) and a quantum yield that is only about 20% as high. 4 DAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques.