Ligation of DNA is a critical step in many modern molecular biology workflows. The sealing of nicks between adjacent residues of a single-strand break on a double-strand substrate and the joining of double-strand breaks are enzymatically catalyzed by DNA ligases.

This is accomplished by covalently connecting the sugar backbone of the two DNA fragments. This reaction, called ligation, is performed by the T4 DNA ligase enzyme. The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join the nucleotides together.

Ligation involves joining up the ends of a DNA with other ends, however, each DNA fragment has two ends, and if the ends are compatible, a DNA molecule can circularize by joining its own ends. At high DNA concentration, there is a greater chance of one end of a DNA molecule meeting the end of another DNA, thereby forming intermolecular ligation.

DNA ligation is the process of joining two strands or fragments of DNA molecules through a phosphodiester bond formed by the enzyme DNA ligase. DNA ligase is involved in maintaining the structural integrity of genomes by participating in DNA replication or repair processes.

2023年8月3日 · DNA ligase is an enzyme that catalyzes the ligation reaction, ligation is the process of joining various types of DNA fragments. DNA ligase joins the DNA molecule covalently by catalyzing the formation of phosphodiester bonds between adjacent nucleotides.

2024年11月16日 · DNA ligation is a fundamental technique in molecular biology that involves covalently joining two DNA fragments together. It is performed by ligase, an enzyme that catalyzes the formation of a phosphodiester bond between the 3' hydroxyl group of one fragment and the 5' phosphate group of the other.

DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. The DNA ligation kit contains the reagents necessary to increase the consistency of ligations.

2023年9月6日 · DNA ligase is an enzyme that catalyzes the formation of a covalent bond between a 5’ phosphate and a 3’ hydroxyl group at the termini of DNA fragments; this bond ligates the two DNA fragments together.

2024年12月27日 · DNA ligation reactions are fundamental processes in molecular biology, enabling the joining of DNA fragments to form recombinant DNA molecules. These reactions utilize specific ends created by restriction enzymes, leading to two primary types of ligation: blunt-end ligation and sticky-end ligation.

DNA ligases are used chiefly to create novel combinations of nucleic acid molecules and to attach them to vectors before molecular cloning. They are either of bacterial origin or bacteriophage encoded and have different properties, as discussed here.