A cryoprotectant is a substance used to protect biological tissue from freezing damage (i.e. that due to ice formation). Arctic and Antarctic insects, fish and amphibians create cryoprotectants (antifreeze compounds and antifreeze proteins) in their bodies to minimize freezing damage during cold winter periods. Cryoprotectants are also used to ...

Cryoprotectants are one of the important factors in cryopreservation, and they are currently an active research area. The ideal cryoprotectant should possess high water solubility, permeability, low toxicity, non-reactivity, and no precipitation at high concentrations.

dissolved molecules prevent water from freezing completely. With cryoprotectant, the percentage of cryoprotectant present in solution increases as ice grows. At any given tem-perature, ice growth stops when the cryoprotectant becomes concentrated enough to make the melting point equal to the surrounding temperature. Eventually the cryoprotec-

Whether the mechanical or osmotic effects of freezing dominate, the end result is the same; unprotected cooling and thawing of cells is a process incompatible with life. To mitigate these effects, two protective actions must be carried out: use of a cryoprotectant, and selection of an appropriate cooling and thawing rate. Permeating Agents

2024年5月21日 · A cryoprotectant is a chemical compound that limits damage to biological tissues in freezing conditions. Without protection, cells will rupture when they freeze as a result of expanding water, causing severe injury or death to living organisms, and ruining tissue samples or frozen food products.

Peroxidation due to cryoprotectant treatment is a vital factor for cell survival in Arabidopsis cryopreservation. Plant Sci 2013;212:37–47 [ DOI ] [ PubMed ] [ Google Scholar ] 89.

2017年6月1日 · The definition of a cryoprotectant is any solute which, when added to cells in their medium, allows higher post-thaw recoveries than if it were not present [143]. However, before discussing the nature of CPAs and these applied definitions, it is worth considering the challenges that are imposed by cooling to ultra-low temperatures — i.e. the ...

2022年7月18日 · A major challenge with this method is cryoprotectant toxicity and the need for rapid but stepwise removal of the CPA to avoid post-thaw toxicity and osmotic damage.

Cryopreservation has become a central technology in many areas of clinical medicine, biotechnology, and species conservation within both plant and animal biology. Cryoprotective agents (CPAs) invariably play key roles in allowing cells to be processed for storage at deep cryogenic temperatures and t …

Cryoprotectant addition has become a rather mundane step within the overall protocol. However, for future advances in cryobiology and to meet new challenges in the clinical use of cryopreserved cells or tissues, it will be essential to have an understanding of the development and current status of the biological and chemical knowledge on ...