Light-Induced Rescue of Breathing after Spinal Cord Injury
2008年11月12日 · A model of ChR2 activation that can lead to long-lasting recovery of muscle activity after spinal cord injury One component that plays an important role in activity-dependent synaptic plasticity, learning, and adaptation in the CNS is the glutamatergic NMDA receptor.
Improving hindlimb locomotor function by Non-invasive AAV …
2021年2月3日 · By taking advantage of the compromised blood-spinal cord barrier following SCI, we optimized a set of procedures in which AAV9 vectors administered via the tail vein efficiently transduce...
Cortico–reticulo–spinal circuit reorganization enables functional ...
2018年3月19日 · After a spinal cord injury (SCI), only half of affected individuals regain supraspinal control of movements below the level of the lesion.
Light-induced rescue of breathing after spinal cord injury
2008年11月12日 · After cervical SCI, respiratory deficits can result through interruption of descending presynaptic inputs to respiratory motor neurons in the spinal cord. Expression of channelrhodopsin-2 (ChR2) and photostimulation in neurons affects neuronal excitability and produces action potentials without any kind of presynaptic inputs.
Corticospinal populations broadcast complex motor signals to ...
2021年11月4日 · For injections of AAV-retro-GCaMP6f, AAV-retro-Cre.mCherry, AAV-retro-ChR2.tdTomato or AAV-retro-FlpO, one penetration was made into each segment of the spinal cord between C3 and C7.
Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord …
2018年3月3日 · CNS injury often severs axons. Scar tissue that forms locally at the lesion site is thought to block axonal regeneration, resulting in permanent functional deficits. We report that inhibiting the generation of progeny by a subclass of pericytes led to decreased fibrosis and extracellular matrix deposition after spinal cord injury in mice.
A Latent Propriospinal Network Can Restore Diaphragm Function …
2017年10月17日 · Spinal cord injury (SCI) above cervical level 4 disrupts descending axons from the medulla that innervate phrenic motor neurons, causing permanent paralysis of the diaphragm. Using an ex vivo preparation in neonatal mice, we have identified an excitatory spinal network that can direct phrenic motor bursting in the absence of medullary input.
Channelrhodopsin-2 Assisted Circuit Mapping in the Spinal Cord …
2022年5月27日 · (a) ChR2 expression in dorsal horn neurons can be achieved by viral injection either directly into the spinal cord to transduce dorsal horn neurons, or into the brain to transduce projection neurons via retrograde transport. Importantly this can be done using wild type animals and a pan neuronal virus to drive expression in all neurons at the ...
Chemogenetic silencing of spinal cord-projecting cortical neurons ...
2022年8月1日 · In this study, we showed for the first time that Thy1-ChR2-EYFP mice are a useful tool, in terms of the expression pattern of ChR2 in the DRG, photostimulation-evoked behavioral and neuronal responses, and resistance to the effects of morphine.
Nerve injury disrupts temporal processing in the spinal cord …
2024年2月27日 · How mechanical allodynia following nerve injury is encoded in patterns of neural activity in the spinal cord dorsal horn (DH) remains incompletely understood. We address this in mice using the spared nerve injury model of neuropathic pain …