Dissecting cell membrane tension dynamics and its effect on …
2024年5月27日 · Here, we report a force-controlled nanopipette-based method that combines fluidic force microscopy with fluorescence imaging for precise manipulation of the cellular membrane tension while...
Force spectroscopy of single cells using atomic force microscopy …
2021年9月23日 · In this Primer, we provide a comprehensive overview of the use of AFM-based force spectroscopy (AFM-FS) to study the strength and dynamics of cell adhesion from the cellular to the...
Atomic Force Microscopy for Cell Membrane Investigation
2019年5月31日 · Atomic force microscopy (AFM) is a very versatile tool for studying biological samples at nanometer-scale resolution. The cell membrane plays a key role in compartmentalization, nutrient transportation, and signal transduction, while the structural feature of both sides of the membrane remains elusive. Here we describe our methods for the ...
The structure and function of cell membranes examined by …
2015年4月20日 · This review summarizes the new progress regarding membrane structure using in situ AFM and single-molecule force spectroscopy (SMFS), which may shed light on the study of the structure and functions of cell membranes.
Atomic force microscopy-based mechanobiology - Nature
2018年11月1日 · A promising AFM-based approach to measuring the elasticity, viscosity and coupling of the cell membrane to the actomyosin cortex is to mechanically extract cell membrane tethers...
Studying structure and functions of cell membranes by single …
For cell membrane imaging, the AFM imaging could provide higher resolution to visualize the arrangement of proteins and the lipid bilayer of cell membranes at the molecular level, but shows disadvantages in identifying the proteins. However, super-resolution microscopy is good at identifying the proteins by fluorescence labeling, although with ...
Atomic Force Microscopy: A Nanoscopic Application in Molecular and Cell ...
2018年11月4日 · At the prosperous fields of nanoscience and biology, the nanoscale analysis of cells and cell membrane using atomic force microscopy (AFM) is an exciting and rapidly developing research area. Over the past decade, there has been tremendous progress in the use of AFM to observe living cells and membrane proteins at high resolution.
Atomic force microscopy: a nanoscopic window on the cell surface
2011年6月10日 · Because of its outstanding signal-to-noise ratio, AFM can directly image single membrane proteins and live cells at nanometre resolution in buffer solution and at ambient temperature, which is a key advantage over electron- and light-microscopy techniques.
Visualizing intracellular nanostructures of living cells by
2021年12月22日 · Here, we overcome this limitation by nanoendoscopy-AFM, where a needle-like nanoprobe is inserted into a living cell, presenting actin fiber three-dimensional (3D) maps, and 2D nanodynamics of the membrane inner scaffold, resulting in …
Atomic Force Microscopy in Molecular and Cell Biology
Atomic force microscopy (AFM) can be used to detect the superstructures of the cell membrane, cell morphology, cell skeletons and their mechanical properties. Opening up new fields of in-situ dynamic study for living cells, enzymatic reactions, fibril growth and biomedical research, these combined techniques will yield valuable new insights ...