What is CRISPR/Cas9? - PMC - PubMed Central (PMC)
CRISPR/Cas9 is a gene-editing technology which involves two essential components: a guide RNA to match a desired target gene, and Cas9 (CRISPR-associated protein 9)—an …
Genome engineering using the CRISPR-Cas9 system - Nature
2013年10月24日 · The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to …
CRISPR/Cas9 therapeutics: progress and prospects
2023年1月16日 · After a series of processing and maturation processes to generate a single guide RNA (sgRNA), the sgRNA guides Cas9 to shear the DNA strand that disrupts the …
Explainer: How CRISPR works - Science News Explores
2017年7月31日 · CRISPR/Cas9 in its original form is a homing device (the CRISPR part) that guides molecular scissors (the Cas9 enzyme) to a target section of DNA. Together, they work …
Past, present, and future of CRISPR genome editing technologies
2024年2月29日 · CRISPR genome editing relies on RNA-guided nucleases such as Cas9 and Cas12a for site-specific target DNA recognition and cleavage. Cas9 utilizes a dual-guide RNA …
CRISPR-Cas: biology, mechanisms and relevance - PMC
The ability of the minimal CRISPR-Cas9 system to target DNA sequences using programmable RNAs has opened new avenues in genome editing in a broad range of cells and organisms …
Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA ...
2023年1月13日 · Here we review the development of CRISPR-Cas9/Cas12/Cas13 nucleases, DNA base editors, prime editors, and RNA base editors, focusing on the assessment and …
A Review on the Mechanism and Applications of CRISPR/Cas9…
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system has altered life science research offering enormous options in …
Development and Applications of CRISPR-Cas9 for Genome …
2014年6月5日 · Derived from a microbial defense system, Cas9 can be guided to specific locations within complex genomes by a short RNA. The development, applications, and future …
Principles of CRISPR-Cas9 technology: Advancements in genome …
2024年2月1日 · The CRISPR-Cas9 system comprises two fundamental components: the Cas9 protein and the guide RNA (gRNA). Functioning as a molecular scissor, Cas9 cleaves the …