Markers of microglia in post-mortem brain samples from patients …
2017年12月12日 · Expression-based assays like qPCR, western blot, or intensity of immunohistochemistry staining indicate the amount of Iba1 in a sample. An increase could reflect a change in cell numbers, cell ...
TREM2-dependent activation of microglial cell protects ... - Nature
2024年8月26日 · Following MNU treatment, Trem2−/− mice showed exacerbated photoreceptor cell death, decreased microglia migration and phagocytosis, reduced microglial PPARγ activation and CD36 expression.
CD36 neutralisation blunts TLR2-IRF7 but not IRF3 pathway in
2023年2月9日 · Treatment with CD36 neutralizing antibody reduced LPS-mediated increase of CD86 (Fig. 2d–f) (p < 0.0001) and MHCII (p = 0.0012) (Fig. 2j–l) immunoreactivity in Iba1 + cells.
CD36 deletion prevents white matter injury by modulating …
2024年6月5日 · Immunofluorescence staining exhibited robust CD36 expression in astrocytes and microglia following TBI. Genetic deletion of CD36 ameliorated TBI-induced white matter injury, as evidenced by a reduced SMI-32/MBP ratio and G-ratio.
SELENOK-dependent CD36 palmitoylation regulates microglial …
2024年4月1日 · SELENOK-dependent CD36 palmitoylation in macrophages significantly influences CD36 stability. This interplay places CD36 at a juncture directly connected with the functionality of SELENOK and microglial Aβ phagocytosis, positioning it as a strategic target for mechanistic exploration.
Isolation driven changes in Iba1-positive microglial morphology …
Using morphological measures of Iba1 to index microglial intensity, complexity, and process length, we identified different effects of brief isolation on microglial complexity in the basal region of the amygdala between adults and adolescents alongside overall increases in intensity of Iba1 in several cortical brain regions.
Silencing the Cytoskeleton Protein Iba1 (Ionized Calcium …
In this study we showed that by silencing Iba1, a cytoskeleton protein acting as an actin-cross linking protein specifically expressed in microglia and macrophages, the migration, proliferation and cell adhesion of microglia BV2 cells were significantly reduced.
Improving outcomes in intracerebral hemorrhage through …
2023年10月1日 · Our study reveals that, 1) PSL-IL10 treatment resulted in significantly improved outcomes and accelerated hematoma resolution in the acute phase of ICH; 2) PSL-IL10 inhibited glial activation and down-regulated pro-inflammatory cytokine production; 3) PSL-IL10 induced Iba1 + cells with a stronger phagocytosis ability; 4) PSL-IL10 activated ...
CD36-mediated uptake of myelin debris by macrophages and …
2020年7月27日 · We show that CD36 protein levels are significantly elevated in myelin-containing phagocytes in vitro and in vivo. CD36 protein level was primarily regulated through the NRF2 signaling pathway. Pharmacological inhibition of CD36 reduced the uptake of myelin debris and promoted neuroinflammation in vitro and in vivo.
Scavenger receptor CD36 governs recruitment of myeloid cells to …
2022年2月11日 · In neonatal mice with intact or globally disrupted CD36 signalling (CD36 KO), we characterized the phenotypes of myeloid cells by flow cytometry and the underlying gene expression signatures in the CPs contralateral and ipsilateral to tMCAO by RNA sequencing analyses, focussing on early post-reperfusion time window.