Different methods of detaching adherent cells and their effects on …
2022年4月5日 · The enzymes trypsin and accutase (Accutase ®, Innovative Cell Technologies) are frequently used for detaching adherent cells. Trypsin cleaves peptides after lysine or arginine residues that...
Accutase replacement by trypsin? - ResearchGate
However, over the past decade+, many people have found that Accutase works very well for certain cell types that may not be detached well by Trypsin. Accutase is a cocktail of...
Different methods of detaching adherent cells and their effects on …
2022年4月5日 · A mild cell detachment buffer, accutase, is recommended for the replacement of trypsin to dissociate adherent cells and thereby avoid cellular damage. In this study, we demonstrated that use of accutase for cellular detachment may …
Compared with ethylenediaminetetraacetic acid (EDTA)‐based nonenzymatic cell dissociation bufers, accutase was associated with significant decreases in the surface Fas ligands and Fas receptors....
Efficient propagation of single cells Accutase-dissociated human ...
This study demonstrates that hESCs can be passaged as single cells using Accutase, a formulated mixture of digestive enzymes. In contrast to trypsin treatment, Accutase treatment does not significantly affect the viability and proliferation rate …
Macrophage retrieval from 3D biomaterials: A detailed …
2021年2月1日 · To address this, we compared the efficiency of human PBMC-derived MDMs dissociation with the following methods: 1) EDTA with and without cold shock, 2) trypsin, 3) Accutase, 4) a commercially available macrophage detachment solution and 5) cell scraping.
Impact of Detachment Methods on M2 Macrophage Phenotype and Function
Our data demonstrate that while exposure to trypsin or accutase yields highly efficient recovery of viable cells, such chemical cleavage results in loss of select M2 cell surface markers with correlative changes in cell function.
Mesenchymal stem cell detachment with trace trypsin is superior …
2017年3月11日 · Trypsin is frequently used to dissociate mesenchymal stem cells (MSCs) for in vitro adhesion and chemotaxis assays. However, its potential impact on surface receptor degradation is poorly understood. The purpose of this study was to evaluate the effect of trypsin-EDTA exposure versus PBS-EDTA on MSC surface receptor integrity and function.
Accutase is an alternative enzyme mixture formulated with proteolytic and collagenolytic enzyme activity. Accutase solution does not contain mammalian or bacterial-derived products and is typically considered gentler than Trypsin.
TrypLE Express Enzyme use for cell dissociation: is it worth it?
2022年5月16日 · Wondering if TrypLE Express cell dissociation agent is significantly better than other options for culturing mammalian cell lines. Currently mainly using Accutase (for fibroblasts and epithelial...