2020年5月4日 · To address this gap, we have developed an open-source suite of 14 optimized bioluminescence resonance energy transfer (BRET) Gαβγ biosensors (named TRUPATH) to …

2022年6月27日 · Here, we exploit the remarkable sensing capacity of G-protein coupled receptors to construct yeast-based biosensors for real-life applications. To establish proof-of-concept, …

2024年4月30日 · In a recent study published in Cell, Janicot et al. introduced a universal biosensor platform capable of detecting GPCR signaling at endogenous levels, which …

2024年3月14日 · Here, we developed a collection of compact ONE vector G-protein Optical (ONE-GO) biosensor constructs as a scalable platform that can be conveniently deployed to …

2022年9月28日 · We will introduce fluorescent GPCR biosensors based on a variety of strategies such as fluorescent resonance energy transfer (FRET), bioluminescence resonance energy …

A hallmark of heterotrimeric G protein activation by GPCRs is the rearrangement or dissociation of the Gα and Gβγ subunits. The most consistent design format for Gα/Gβγ-based GPCR …

We construct a CB 2 receptor-based biosensor, optimize it to achieve high sensitivity and dynamic range, and prove its effectiveness in three applications of increasing difficulty. First, we screen …

2024年1月2日 · Here, we developed a collection of compact ONE vector G-protein Optical (ONE-GO) biosensor constructs as a scalable platform that can be conveniently deployed to …

2025年1月15日 · The Borealis arrestin biosensor (Montana Molecular) also relies on a conformational change in the β-arrestin, with recruitment of the biosensor to the GPCR …

2024年3月14日 · Here, we developed a collection of compact ONE vector G-protein Optical (ONE-GO) biosensor constructs as a scalable platform that can be conveniently deployed to …