2018年7月20日 · Using immunolabelling and optogenetics combined with in vitro electrophysiology, we investigated the possible presence and subcellular regulation of hippocampal co-transmission of acetylcholine...

Based on PSC latency and pharmacological sensitivity, our results suggest monosynaptic release of both GABA and ACh. Anatomical analysis showed that forebrain cholinergic neurons express the GABA synthetic enzyme Gad2 and the vesicular GABA transporter (Slc32a1).

Functional evidence for cotransmission of GABA with Acetylcholine is reviewed from retinalstarburst amacrine cells and cortical cholinergic fibers. Colabeling of GABAergic markers indicates that much of the forebrain cholinergic neurons mayalso release GABA

2016年1月1日 · We review functional evidence for cotransmission of GABA with acetylcholine. Colabeling of GABAergic markers indicates that most cholinergic neurons may also release GABA. Optogenetic stimulation will allow determination of cholinergic cells that release GABA.

2015年2月27日 · We confirmed the direct release of GABA by knocking out Slc32a1 from cholinergic neurons. Our results identify GABA as an overlooked fast neurotransmitter utilized throughout the forebrain cholinergic system. GABA/ACh corelease may have major implications for modulation of cortical function by cholinergic neurons.

Acetylcholine (ACh) was the first identified neurotransmitter and is synthesized in nerve terminals from the precursors acetyl-CoA and choline, in a reaction catalyzed by choline acetyltransferase (ChAT) (figure 2.1). After synthesis in the cytoplasm of the neuron, a vesicular ACh transporter (VAChT) loads ACh into each cholinergic vesicle.

2018年7月20日 · We demonstrate that these synapses do not co-release but co-transmit GABA and acetylcholine via different vesicles, whose release is triggered by distinct calcium channels. This co-transmission evokes composite postsynaptic potentials, which are mutually cross-regulated by presynaptic autoreceptors.

2020年7月2日 · We show that the nearly all cortical ChAT + neurons in mice are specialized VIP + interneurons that release GABA strongly onto other inhibitory interneurons and acetylcholine sparsely onto layer 1 interneurons and other VIP + /ChAT + interneurons.

2020年7月2日 · We show that the nearly all cortical ChAT + neurons in mice are specialized VIP + interneurons that release GABA strongly onto other inhibitory interneurons and acetylcholine sparsely onto layer 1 interneurons and other VIP + /ChAT + interneurons.

2016年1月1日 · We review functional evidence for cotransmission of GABA with acetylcholine. Colabeling of GABAergic markers indicates that most cholinergic neurons may also release GABA. Optogenetic stimulation will allow determination of cholinergic cells that release GABA.