5-Bromo-2′-deoxyuridine (BrdU) is a halogenated pyrimidine that can be incorporated into newly synthesized DNA during the S phase of the cell cycle. BrdU is widely used in fate-mapping studies of embryonic and adult neurogenesis to identify newborn neurons, however side effects on neural stem cells and their progeny have been reported.

Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU immunohistochemistry has been instrumental for the study of the development of the nervous syste …

2025年2月6日 · After performing immunofluorescent labeling for BrdU and neuronal markers (including NeuN, calbindin and NSE), we detected double-labeled cells — evidence of newborn neurons. ... Spalding et al ...

2007年1月1日 · BrdU can be revealed by immunohistochemistry using a monoclonal antibody directed against single-stranded DNA containing BrdU. BrdU immunohistochemistry has been instrumental to confirm that neurogenesis occurs in the adult mammalian brain, including in human, and is currently the most used technique for studying adult neurogenesis.

Birth dating neurons with bromodeoxyuridine (BrdU) labeling is an established method widely employed by neurobiologists to study cell proliferation in embryonic, postnatal, and adult brain.

BrdU is widely used in fate-mapping studies of embryonic and adult neurogenesis to identify newborn neurons, however side effects on neural stem cells and their progeny have been reported. In vivo astrocyte-to-neuron (AtN) conversion is a new approach for generating newborn neurons by directly converting endogenous astrocytes into neurons.

5-Bromo-2′-deoxyuridine (BrdU) is frequently used as a mitotic marker in studies of cell proliferation. Recent studies have reported cytotoxic effects of BrdU on neural progenitor cells in embryonic and neonatal brains in vivo and in adult tissue studied in vitro.

2007年1月1日 · BrdU immunohistochemistry has been instrumental for the study of the development of the nervous system, and to confirm that neurogenesis occurs in the adult mammalian brain, including in human. However, the use of BrdU for studying neurogenesis is not without pitfalls and limitations. BrdU is a toxic and mutagenic substance.

2006年11月2日 · Neurogenesis within the adult central nervous system is demonstrated using an exogenous cell tracer, 5'-bromo-2'-deoxyuridine (BrdU), in combination with endogenous neuronal markers.

A promise of AtN conversion is to generate new neurons from disease-responsive reactive astrocytes. These reactive astrocytes can be prelabeled with BrdU and examined for BrdU-traced neurons after AtN conversion.