2003年7月1日 · We isolated a zebrafish homologue of EGFR (egfr) that shows a 63% amino acid overall identity to human EGFR but with 90% amino acid identity in the kinase domain. Whole mount in situ hybridization showed ubiquitous distribution of egfr transcripts during gastrulation, somitogenesis and later stages.

2022年1月17日 · In this study, we created zebrafish mutants for Egf ( egf -/- ) and Egfr ( egfra -/- and egfrb -/- ) by CRISPR/Cas9 technique. Surprisingly, these mutants all survived well with little abnormality in growth and development. Spermatogenesis and folliculogenesis were both normal in egf -/- males and females.

2022年1月16日 · Using CRISPR/Cas9-mediated gene knockout approach, we investigated the functional importance of Egf/egf and Egfr (egfra and egfrb) in the zebrafish with particular emphasis on their roles in reproductive performance, especially gonadal development and function. Our data demonstrated that EGF was dispensable for zebrafish reproduction despite ...

2014年1月15日 · Here we used the zebrafish to define several developmental defects caused by impairment of Egf signaling, a major pathway of interest in tumor biology. We inactivated Egf signaling by genetically blocking Egf expression or using …

2024年4月16日 · Wnt9a signals through Fzd9b, the co-receptor LRP5 or LRP6 (LRP5/6), and the epidermal growth factor receptor (EGFR) to promote early proliferation of zebrafish and human hematopoietic stem cells during development.

2023年4月16日 · Our results demonstrated that EGFR was activated in hepatocytes from high fructose (HF)-induced NAFLD zebrafish and interfered with Mfn1 polymerization, leading to reduction of MtDNA. Suppression of EGFR activation or mitochondrial translocation significantly improved mitochondrial morphology and increased mitochondrial DNA, ultimately ...

2004年9月1日 · In the present study, we cloned full-length cDNAs for epidermal growth factor (EGF), EGF receptor (EGFR), and three truncated forms of EGFR (EGFR15, 12, and 8) from the zebrafish ovary. Zebrafish EGF was predominantly expressed in the ovary and testis, while EGFR and its truncated forms were highly expressed in all tissues examined except the ...

In the zebrafish liver injury model, both hepatocytes and biliary epithelial cells contributed to LPCs. EGFR inhibition promoted the differentiation of LPCs regardless of their origin. Notably, short-term treatment with EGFR inhibitors resulted in better liver recovery over the long term.

Using the zebrafish, a diurnal vertebrate, to investigate the genetic regulation of sleep, we found that epidermal growth factor receptor (EGFR) signaling is necessary and sufficient for normal sleep levels and is required for the normal homeostatic response to sleep deprivation.

2024年8月26日 · This prompted in vitro investigation of the effects of Socs4b on EGFR signaling. Stimulation with EGF resulted in enhanced levels of pEGFR and pERK in cells transfected with Socs4b WT, but no change in those transfected with Socs4bΔ18. This suggests that zebrafish Socs4b WT can also regulate EGFR signaling, but not the truncated form.