A warning to area residents to avoid contact with local streams came several hours after officials responded to the spill.

2 | Transfer the homogenate to a fresh tube and sequentially add 0.1 ml of 2 M sodium acetate (pH 4.0), 1 ml of phenol and 0.2 ml of 49:1 (wt/vol) chloroform/isoamyl alcohol per milliliter of ...

"As a result of the compromised pipe, we have found low amounts of caustic and phenol and trace amounts of butyl acetate," the statement said. "The fire department conducted air monitoring along ...