RPE1 cells were grown on glass coverslips to 70-80 % confluency and serum-starved for 24 h to induce ciliogenesis. Cells were fixed with 3% PFA in PBS for 15 min and permeabilized for 5 min in 0.2% ...

In this infographic, we compare two methods for tracking cellular metabolism – periodic sampling and continuous monitoring – before highlighting when continuous monitoring can benefit your research ...

Structural Genomics Consortium, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States ...

The ECM (extracellular matrix) provides the microenvironmental niche sensed by resident vascular smooth muscle cells (VSMCs). Aging and disease are associated with dramatic changes in ECM composition ...

The surface antigen CD14 plays an important role in innate immunity, serving as a pattern recognition receptor for lipopolysaccharides (LPS). The aim of this study was to investigate the proliferation ...

Several diseases affect the alveoli, and the efficacy of medical treatments and pharmaceutical therapies is hampered by the lack of pre-clinical models able to recreate in vitro the diseases.

When the cells reached 70%–80% confluency, they were infected with JEV at an MOI of 0.1 and incubated for 72 h. The cell morphology was observed during the incubation period, and uninfected cells were ...