Two-photon excitation (TPE) microscopy enables imaging of living tissues up to a depth of one millimeter, which is 6 to 10-fold deeper than with confocal microscopy. Like confocal microscopy, TPE ...

the design of spinning disks for two-photon excitation 27 or the use of fast acousto-optic scanning methods. Also, new phase-shaping methods (scanning-less microscopy 28) can enable the ...

ifrec.osaka-u.ac.jp During the past decade, multi-photon or ‘two-photon’ excitation microscopy has launched a new era in the field of biological imaging. The near-infrared excitation laser for ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

As the optical set-up of STED microscopy is based on a laser scanning microscopy (LSM) system, it potentially has several merits of LSM like confocal or two-photon excitation LSM. In this article, we ...

Layered structure depth profile images of liquid crystal samples between two cover slips obtained by the three photon excited fluorescence technique. Experimental setup for the multimodal nonlinear ...

The Ultima IV is a fully automated upright microscope – objectives are above the stage. This configuration is ideal for in vivo imaging of whole specimens. The Ultima IV is equipped with a tunable two ...

The two-photon side of the microscope provides the optical pathway for guiding the excitation laser light from the table up into the scanning galvanometric mirrors and then expanding the beam ...

fixed stage upright fluorescence microscope Three single photon excitation laser lines, 488nm, 561nm and 647 nm Coherent Chameleon Ultra II ultrafast laser for two-photon excitation Tunable from 680nm ...

The innovative microscope, named DEEPscope, combines two-photon and three-photon microscopy ... Key to this advancement is DEEPscope's adaptive excitation system and multi-focus polygon scanning ...

Two-photon microscopy can image deep into scattering ... This is critical for the subsequent high-speed imaging with the ...

Fluorescence microscopy can be realized by making use of two-photon excitation (TPE) of fluorescent molecules. This has the distinct advantage over single-photon excitation in that the sample is ...