Two-photon excitation (TPE) microscopy enables imaging of living tissues up to a depth of one millimeter, which is 6 to 10-fold deeper than with confocal microscopy. Like confocal microscopy, TPE ...

the design of spinning disks for two-photon excitation 27 or the use of fast acousto-optic scanning methods. Also, new phase-shaping methods (scanning-less microscopy 28) can enable the ...

a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence excitation only with the diffraction limited spot of the objective lens, thus creating an inherent ...

A miniature head-mounted two-photon microscope small enough ... All the microscope optics reside in the miniature microscope, while the excitation and emission light are delivered and collected ...

The Ultima IV is a fully automated upright microscope – objectives are above the stage. This configuration is ideal for in vivo imaging of whole specimens. The Ultima IV is equipped with a tunable two ...

Layered structure depth profile images of liquid crystal samples between two cover slips obtained by the three photon excited fluorescence technique. Experimental setup for the multimodal nonlinear ...

The two-photon side of the microscope provides the optical pathway for guiding the excitation laser light from the table up into the scanning galvanometric mirrors and then expanding the beam ...

Two-photon microscopy can image deep into scattering ... This is critical for the subsequent high-speed imaging with the ...

The innovative microscope, named DEEPscope, combines two-photon and three-photon microscopy ... Key to this advancement is DEEPscope's adaptive excitation system and multi-focus polygon scanning ...