The screened colonies were confirmed to be single colonies by multiple purification cultures and microscopic examinations, and they were stored in liquid Luria-Bertani (LB) medium (tryptone 10 g/L, ...

Aniline blue medium: tryptone 10 g, NaCl 10 g, yeast extract 5 g, agar 18 g, deionized water 1 L, adjust pH = 7.0, sterilized at 121°C for 30 min. 0.1 g of aniline blue was added when the temperature ...

The swab will be transferred to a neutralising agent (containing 30 g/L Tween 80, 3 g/L lecithin, 1 g/L histidine, 5 g/L sodium thiosulphate, 8.5 g/L sodium chloride and 1 g/L tryptone) to nullify ...

The journey to develop an upstream bioprocess is complex, involving process development, optimization and scale-up. Hence, it’s important to choose the right equipment to accelerate this journey while ...