Inhibition of nonsense-mediated decay turns immunologically cold tumors hot by increasing the amount of mutant RNA leading to an increase in T-cell-targetable neoantigens on the cell surface.

Vero (ATCC, CCL-81) cells were obtained from the China Center for Type Culture Collection (Wuhan, China) and were cultured in DMEM containing 10% fetal bovine serum (FBS, Gibco, Waltham, MA, USA).

The wild-type PEDV strain GX4/2021 (GenBank accession number OP382083) was grown on Vero cells using a maintenance medium, DMEM supplemented with 2 μg/ml of trypsin (Gibco, USA), 0.3% tryptose ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. The fast and transient induction of immediate ...

The resulting cell suspension was centrifuged at 1000 rpm for 3 min, and the pellet was further digested with 0.25% Trypsin-EDTA (Gibco, 25200072) at 37℃ for 10 min. The digestion was terminated by ...

Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. Engineered extracellular vesicles play an increasingly important role in the treatment ...

Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Gaoke Innovation Center, Guangming District, Shenzhen, Guangdong 518132, P. R. China ...