Both groups received intraperitoneal injection of cerulein (100 mg/kg/h x12) to induce AP. H&E and TUNEL staining of pancreas were performed to assess histology and cell death. To measure vascular ...

Finally, the samples were stained with DAPI, mounted with FLUOROMOUNT-G, and imaged under a confocal microscope. TUNEL staining was performed following the manufacturer’s protocol (Roche, 11684795910) ...

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We carried out TUNEL staining according to the manufacturer's instructions (ApopTag Kit; Intergen), except that the DNA fragments which had been labelled with the digoxigenin-nucleotide were ...