A110 is mutated to V110 and then the protein goes out of frame. E. Validation of Orc2 deletion 3 days after Adeno cre transduction. F. Western blot of ORC2 protein 5 days after Adeno cre transduction.

ROSA26 genotyping was carried out with primers F1 forward (common to both alleles ... PCR product was run on 2% agarose gel and WT band was detected at 650bp, and transgenic band at 340bp. Sox2-Cre ...