This work will be of high interest to a broad swathe of scientists studying native macromolecules present at low concentrations in cells. Cryo-EM single-particle analyses typically require target ...

Wash the resin in a disposable standing column with 2 ml of wash buffer and then elute His-tagged protein with wash buffer plus 300 mM imidazole. Problem: We get poor cloning efficiency into ...

(A) Schematic of expression and purification of γ-secretase and C100. (B) Western blot analysis of all components within expressed and purified WT and FAD-mutant γ-secretase complexes, normalized to ...

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This purification process has been carried out using chlorine. In the 1970s, researchers found chlorine could react to organic compounds in water to produce chemical reactions that cause cancer ...

A generated column is a special column that is always computed from other columns. Thus, it is for columns what a view is for tables. There are two kinds of generated columns: stored and virtual. A ...

An index can be defined on more than one column of a table. For example, if you have a table of this form: A multicolumn GiST index can be used with query conditions that involve any subset of the ...

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Purgatory is not a second-chance for damned souls to repent. Instead it is a state of cleansing and purification for souls destined for heaven. Also Purgatory is not a means to earn our way to ...