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25 天
基因工厂云探秘系列4-- 从PCR到基因组装:揭示合成关键步骤与精准验证
酶促填充法基于聚合酶循环组装(Polymerase Cycling Assembly,PCA)技术,使用DNA聚合酶将重叠的寡核苷酸延伸成双链片段,通过overlap PCR获取较长片段,然后将各片段一起组装至载体上,最终构建出完整载体。
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