Real-time PCR is the favored method for measuring gene ... to detection with SYBR Green I in its simplicity, but allows multiplexing. To help researchers configure single- or multiplex assays ...

We assessed six cell lines known to harbor Nras gene mutations using our multicolor multiplex assay. Two of the cell lines, MOLT-4 and PA-1, contain mutations in codon 12, while the remaining four ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...

Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount of DNA present in a sample. The procedure requires specialized thermal cyclers with ...

real-time PCR (qPCR), digital PCR (dPCR), reverse transcription PCR (RT-PCR), hot-start PCR, multiplex PCR, and other PCR techniques. The qPCR segment holds the largest share of the market.

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

The new capabilities overcome the assay design challenges of currently available quantitative multiplex PCR methods, making QIAcuity digital PCR ideal for applications such as translational ...

Real-Time PCR (qPCR) market Insights: Used extensively in diagnostics, life sciences, and environmental testing ...

QIAcuity digital PCR now offers ability for simultaneous detection of up to 12 targets from one biological sample, a more than two-fold increase from prior capability ...