The amount of GFP protein (P) produced was quantified by western blot using rabbit anti-GFP antisera. The GFP fluorescence (F) was quantified by fluorescence spectroscopy of whole-cell lysates.
Here we describe the generation and characterization of green fluorescent protein (GFP)-based substrates ... and incubated for 1 h with rabbit anti-GFP serum (Molecular Probes Europe, Leiden ...