Two-photon excitation (TPE) microscopy enables imaging of living tissues up to a depth of one millimeter, which is 6 to 10-fold deeper than with confocal microscopy. Like confocal microscopy, TPE ...

the design of spinning disks for two-photon excitation 27 or the use of fast acousto-optic scanning methods. Also, new phase-shaping methods (scanning-less microscopy 28) can enable the ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

Layered structure depth profile images of liquid crystal samples between two cover slips obtained by the three photon excited fluorescence technique. Experimental setup for the multimodal nonlinear ...

The Ultima IV is a fully automated upright microscope – objectives are above the stage. This configuration is ideal for in vivo imaging of whole specimens. The Ultima IV is equipped with a tunable two ...

The two-photon side of the microscope provides the optical pathway for guiding the excitation laser light from the table up into the scanning galvanometric mirrors and then expanding the beam ...

A miniature head-mounted two-photon microscope small enough ... All the microscope optics reside in the miniature microscope, while the excitation and emission light are delivered and collected ...

The innovative microscope, named DEEPscope, combines two-photon and three-photon microscopy ... Key to this advancement is DEEPscope's adaptive excitation system and multi-focus polygon scanning ...

Two-photon microscopy can image deep into scattering ... This is critical for the subsequent high-speed imaging with the ...

SpectraPhysics InSight X3 dual laser, including a 680-1300 nm tunable laser and a fixed 1045 nm laser.