T o: Initial number of target gene copies; R o: Initial number of standard gene copies; E: Efficiency of amplification; ct,t: Threshold cycle ... The efficiency of PCR provides information about ...
The mean real-time PCR threshold cycle (Ct) values of all 46 positives were 21 while the mean Ct value of the samples with discordant or invalid results was 20.3 and 25, respectively. The PPA of ...