At 48-72 hours post-transfection, the supernatant (containing the viral particles) is harvested and clarified. At this point lentiviral vectors can be used to transduce the mammalian cell line of ...
Viruses, such as adeno-associated virus and lentivirus, are very efficient ... A potential bottleneck is transfection, where genetic material is introduced into the host cells, leading to the ...
The FDA IND approval of UC101 further showcases VectorBuilder’s mature platform of lentiviral manufacturing. In addition to its extensive FDA IND experience with transfection-based lentiviral ...
541 671 CRISPR cell line (Transient transfection) (Extra order/on-target KO line) 221 274 CRISPR cell line (Lentiviral delivery) (1 ctrl line + 1 on-target KO line) 585 726 CRISPR cell line ...
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