A human intestinal organoid, displaying typical ‘budding crypts’ and a central ‘villus-like’ domain. The main intestinal cell types are shown in green, blue, red and purple. Membranes are ...

Our approach to organoid culture and differentiation involves the use of synthetic matrices to direct morphogenesis through tunable biochemical and mechanical signals. We spatiotemporally pattern ...

12604013; ThermoFisher) per 50 μL BME at 37°C for 10 min and trituration to single cells using a P1000 pipette ... in OGM or washed with Advanced DMEM/F12 and transitioned to differentiation medium ...

Image Processing Pipeline for 2D/3D organoid segmentation - time course samples Image Processing Pipeline for 2D/3D organoid segmentation - time lapse samples Organoid linking algorithm Further ...

The use of intestinal organoid cultures is a foundation for most of the studies in the lab. These cultures are derived from stem cells isolated from human intestinal tissue. The stem cells are ...

is interested in engineering biological tissues to solve heterogeneity and reproducibility challenges in organoid systems. In a recently published Science paper, 1 his team described how they ...

The Stem Cell and Organoid Core currently provides researchers with access to high-quality human induced-pluripotent stem cell (iPSC) production, expansion, validation and quality control services.

Intestinal cells, for example ... the Invasin protein from Yersinia bacteria can be used as key component for organoid culture. Credit: Sensu. Hubrecht Institute. The possibility of culturing ...

The manuscript by Isotani et al characterizes the hyperproliferation of intestinal stem cells (ISCs) induced by nicotine treatment in vivo. Employing a range of small molecule inhibitors, the authors ...