These variables can affect the yield and viability of dissociated cells, necessitating a comprehensive and versatile approach that addresses the unique considerations for fresh tissue dissociation ...
Take the protocol for culturing cortical neurons, for example. To culture cortical neurons, researchers add cell suspensions of dissociated cortex onto a polylysine (PL)-coated substrate and, once the ...
Kelley, Reh, and colleagues (23) performed their assays using dissociated rat retinal cells. These cells were cultured in various amounts of thyroid hormone and/or retinoic acid for either 6 or 14 ...