The resulting experimental mouse is hemizygous for Cre and homozygous for loxP (D). This is the necessary genotype required to conditionally knock out the target gene in the specific tissue. Laura is ...

When Cre recombinase (red circle) is introduced, either as a transgene by crossing into a mouse line carrying the targeted gene locus or on a viral vector, the DNA between the loxP sites (red ...

They used the Cre-loxP system for these experiments, wherein GFP expression in the host cells was prevented by a STOP codon, flanked by the loxP sequence. On adding Cre recombinase, the enzyme ...